pathogenic eskape group bacteria (ATCC)
Structured Review

Pathogenic Eskape Group Bacteria, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 22301 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pathogenic eskape group bacteria/product/ATCC
Average 99 stars, based on 22301 article reviews
Images
1) Product Images from "Restriction of growth and biofilm formation of ESKAPE pathogens by caprine gut-derived probiotic bacteria"
Article Title: Restriction of growth and biofilm formation of ESKAPE pathogens by caprine gut-derived probiotic bacteria
Journal: Frontiers in Microbiology
doi: 10.3389/fmicb.2024.1428808
Figure Legend Snippet: Zone of inhibition of ESKAPE pathogen growth by cell-free supernatant and cell-lysates of LAB isolates. The figure depicts the zone of inhibition radius from agar well diffusion assay. The zone of inhibition was recorded with the zone of inhibition scale (PW297, Himedia) following 24 h of incubation after the addition of sterile-filtered (A) cell-free culture supernatants, and (B) cell lysate of Lactobacillus isolates to the agar-wells. The horizontal bar depicts the Mean (values embedded) of the zone of inhibition radius of three experiments (mm) excluding the diameter of the agar well; Lp. plantarum (MTCC-2621) used as a positive control.
Techniques Used: Inhibition, Diffusion-based Assay, Incubation, Sterility, Positive Control
Figure Legend Snippet: Lactobacillus isolates prevent biofilm formation by ESKAPE pathogens. The biofilm inhibition ability of Lactobacillus isolates was assessed using Cell-free culture filtrate (CSF). The assay was performed in 24-well polystyrene plates. ESKAPE pathogens [ (A) E. faecalis, (B) S. aureus, (C) K. pneumoniae, (D) A. baumannii, (E) P. aeruginosa, and (F) E. coli] were cultured either in the presence or absence of Lactobacillus-CSF and incubated in static conditions at 37 ˚C for 48 hours. Subsequently, quantitative measurement of biofilm was performed using the standard crystal violet-based assay. The bar diagrams depict the Mean ± SD of the absorbance (A 570nm ) values from triplicate wells. The experiment was performed twice. One-way ANOVA was performed to compare the mean differences of each condition compared to the base value without any CSF (negative control biofilm, CB). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. All Lactobacillus -CSF exhibited various degrees of biofilm inhibition on ESKAPE bacteria. Lp plantarum (MTCC-2621) used as a positive control.
Techniques Used: Inhibition, Cell Culture, Incubation, Crystal Violet Assay, Negative Control, Bacteria, Positive Control